| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | cell division cycle 40; EH-binding protein 3; EHB3; pre-mRNA splicing factor 17; PRP17; PRPF17; Cdc40;;CDC40 |
| WB Predicted band size | Calculated MW: 66 kDa ; Observed MW: 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CDC40 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CDC40抗体的3篇文献示例(注：以下内容为模拟生成，非真实文献)：
1. **文献名称**：*CDC40 Antibody Characterization and Its Role in Cell Cycle Regulation*
**作者**：Miller, J. et al.
**摘要**：该研究开发并验证了一种高特异性CDC40多克隆抗体，用于检测哺乳动物细胞中CDC40蛋白的表达水平。通过Western blot和免疫荧光实验，发现CDC40在有丝分裂后期调控中与剪接体复合物相互作用，影响细胞周期进程。
2. **文献名称**：*Monoclonal Antibody Targeting CDC40 Reveals Its Overexpression in Colorectal Cancer*
**作者**：Chen, Y. et al.
**摘要**：报道了一种靶向人CDC40的单克隆抗体的制备及其在结直肠癌组织中的临床应用。该抗体通过免疫组化证实CDC40在肿瘤样本中显著高表达，提示其作为潜在癌症标志物的价值。
3. **文献名称**：*CDC40 Knockdown via Antibody-Mediated Protein Degradation Disrupts mRNA Splicing*
**作者**：Gomez, S. et al.
**摘要**：利用CDC40抗体结合PROTAC技术实现靶向蛋白降解，证明CDC40缺失会导致pre-mRNA剪接缺陷，并触发细胞周期停滞，为癌症治疗提供新策略。
(注：如需真实文献，建议通过PubMed或Google Scholar检索CDC40相关研究，并筛选涉及抗体应用的论文。)
The CDC40 antibody targets the Cell Division Cycle 40 protein (CDC40), a conserved splicing factor critical for pre-mRNA processing and cell cycle progression. CDC40. also known as PRP17. is a core component of the spliceosome, facilitating the removal of introns during RNA splicing. It plays a key role in maintaining genomic stability by ensuring accurate splicing of transcripts involved in DNA repair, mitosis, and checkpoint control. Dysregulation of CDC40 has been linked to cancers, developmental disorders, and cell cycle defects, highlighting its importance in cellular homeostasis.
Antibodies against CDC40 are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in investigations of spliceosome dynamics and cell cycle regulation. Researchers use these antibodies to explore CDC40's role in diseases, particularly its overexpression in malignancies such as colorectal and hepatocellular carcinomas, where it correlates with poor prognosis.
Commercial CDC40 antibodies are typically raised in rabbits or mice, with validation across multiple applications. Their specificity and reliability support mechanistic studies, including how CDC40 mutations or aberrant splicing contribute to pathological conditions. These tools continue to advance understanding of post-transcriptional gene regulation and therapeutic targeting of spliceosome-related disorders.
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