首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素12(IL-12/P40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12/P40含量。IL12/P40是白细胞介素-12的β亚基，属于细胞因子家族，对T细胞和自然杀伤细胞有重要作用。其研究机制涉及与受体的结合和信号转导，对免疫应答和免疫系统稳定至关重要。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域探究感染性疾病、自身免疫疾病或肿瘤微环境中IL-12/P40的动态变化，为免疫调控机制研究、动物疾病模型评估或药物干预效果分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IL-12/P40
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 96-106 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 103 | 97-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3292.2282.2792.07710001.7481.6471.6981.4965001.1521.1311.1420.9402500.7940.7830.7890.5871250.5060.4950.5010.29962.50.4150.4040.4100.20831.250.3080.3030.3060.10400.2020.2010.202 | ||||||
货期：3-5 working days
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