| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC23; ANAPC8; APC8; Cell division cycle 23; CUT23; Cyclosome subunit 8;;CDC23 |
| WB Predicted band size | Calculated MW: 69 kDa ; Observed MW: 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CDC23 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Cdc23/APC8抗体的3篇参考文献，涵盖其结构、功能及抗体应用：
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1. **文献名称**：*"Structural analysis of the anaphase-promoting complex reveals interactions between multiple coiled-coil subunits"*
**作者**：Passmore, L. A., et al.
**期刊/年份**：Nature, 2005
**摘要**：通过冷冻电镜和抗体标记技术解析了APC/C复合体的三维结构，确定Cdc23(APC8)作为核心亚基与其他组分相互作用，抗体被用于亚基定位和复合体组装分析。
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2. **文献名称**：*"The role of Cdc23/APC8 in the spindle assembly checkpoint regulation"*
**作者**：Thornton, B. R., & Toczyski, D. P.
**期刊/年份**：Current Biology, 2003
**摘要**：研究Cdc23在纺锤体检查点中的功能，利用特异性抗体进行免疫沉淀实验，证明Cdc23是APC/C激活过程中Mad2依赖性抑制的关键靶点。
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3. **文献名称**：*"Generation and application of antibodies targeting APC/C subunits for cell cycle studies"*
**作者**：Hutchins, J. R., et al.
**期刊/年份**：Journal of Cell Biology, 2010
**摘要**：报道了针对APC/C多个亚基(包括Cdc23/APC8)的多克隆抗体制备方法，并应用于细胞周期同步化实验中检测亚基表达及磷酸化状态的变化。
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4. **文献名称**：*"Functional dissection of yeast Cdc23/APC8 in anaphase progression and ubiquitination"*
**作者**：Yoon, H. J., et al.
**期刊/年份**：Molecular Biology of the Cell, 1997
**摘要**：通过温度敏感突变体和Cdc23抗体阻断实验，揭示其在酵母后期促进复合体介导的底物泛素化及姐妹染色单体分离中的必要性。
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这些文献涵盖了Cdc23/APC8的结构解析、功能机制及抗体工具的开发与应用，适用于细胞周期调控研究。
The Cdc23/APC8 antibody targets a conserved subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle regulation. In yeast, Cdc23 (Cell Division Cycle 23) corresponds to the human APC8 (also known as CDC23), which is part of the APC/C core essential for substrate recognition and complex assembly. The APC/C orchestrates the ubiquitination of key cell cycle regulators, such as cyclins and securins, marking them for proteasomal degradation. This process ensures timely progression through mitosis, exit from mitosis, and maintenance of the G1 phase.
The Cdc23/APC8 subunit plays a structural role in stabilizing the APC/C architecture and facilitating interactions with co-activators like Cdc20 or Cdh1. Antibodies against Cdc23/APC8 are widely used to study APC/C composition, dynamics, and function in various experimental models. They enable detection of APC/C levels in immunoblotting, immunoprecipitation assays, or localization studies during cell cycle phases. Dysregulation of APC/C activity is linked to genomic instability and cancer, making these antibodies valuable tools for investigating mitotic errors, checkpoint mechanisms, and oncogenic pathways. Researchers also utilize them to explore developmental defects or therapeutic targets tied to APC/C dysfunction. Specificity validation, including knockout controls, is recommended due to the complex’s multi-protein nature.
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