| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC16; ANAPC6; APC6; CDC16 homolog; Cyclosome subunit 6; CDC16Hs; CUT9;;CDC16 |
| WB Predicted band size | 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CDC16 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于 **Cdc16/APC6 抗体**的参考文献示例(文献信息基于学术研究背景综合概括，非真实引用)：
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1. **文献名称**: *"Characterization of the APC6 subunit of the anaphase-promoting complex in human cells"*
**作者**: Smith A, Jones B, et al.
**摘要**: 本研究利用针对 APC6 的多克隆抗体，通过免疫沉淀和 Western blot 技术，验证了 APC6 在人类细胞中的表达及其与其他 APC/C 亚基的相互作用，揭示了其在细胞周期退出中的关键作用。
2. **文献名称**: *"Functional analysis of Cdc16 in yeast APC/C assembly and substrate recognition"*
**作者**: Brown C, Wilson D.
**摘要**: 通过制备酵母 Cdc16 特异性抗体，作者证明了 Cdc16 是 APC/C 结构稳定的必需亚基，并利用抗体阻断实验揭示了其在底物识别中的调控机制。
3. **文献名称**: *"Development of monoclonal antibodies against APC6 for cancer therapeutic studies"*
**作者**: Lee S, Kim H, et al.
**摘要**: 报道了一种新型 APC6 单克隆抗体的开发，该抗体在肿瘤细胞系中显示出特异性结合能力，并通过抑制 APC/C 活性诱导细胞周期停滞，为靶向治疗提供了潜在工具。
4. **文献名称**: *"APC6 phosphorylation regulates mitotic progression in Drosophila"*
**作者**: Garcia R, Patel M.
**摘要**: 利用 APC6 抗体进行免疫荧光染色，研究发现果蝇中 APC6 的磷酸化修饰调控其与 APC/C 的结合能力，进而影响纺锤体组装和染色体分离。
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(注：以上文献为示例性内容，实际引用需以真实发表的论文为准。)
The Cdc16/APC6 antibody targets a conserved subunit of the anaphase-promoting complex/cyclosome (APC/C), a critical E3 ubiquitin ligase regulating cell cycle progression. APC/C orchestrates the ubiquitination and subsequent degradation of key cell cycle regulators, such as securin and cyclin B, to ensure proper transitions between metaphase, anaphase, and mitotic exit. The APC6 subunit (Cdc16 in yeast) is a core structural component of the APC/C complex, contributing to its assembly and stability. It interacts with other subunits like APC3 (CDC27) and APC8 (CDC23) to maintain the complex's architecture and catalytic activity.
Antibodies against APC6/Cdc16 are widely used to study APC/C function, cell cycle dynamics, and mitotic errors. They enable detection of APC6 expression levels, localization, and interactions in various experimental models, including yeast, mammalian cells, and human tissues. Dysregulation of APC/C subunits, including APC6. has been linked to genomic instability, cancer, and developmental disorders. These antibodies are essential tools for investigating mechanisms underlying cell cycle checkpoints, chromosome segregation, and therapeutic targeting of APC/C-related pathologies. Common applications include Western blotting, immunoprecipitation, and immunofluorescence, often validated in species like humans, mice, and *S. cerevisiae*. Host species for these antibodies typically include rabbit or mouse, with polyclonal formats offering broad utility in diverse experimental contexts.
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