| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC45L; CDC45L2; PORC-PI-1; CDC45;;CDC45 |
| WB Predicted band size | Calculated MW: 66 kDa ; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CDC45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于CDC45抗体的参考文献(示例为虚构，仅供格式参考)：
1. **文献名称**: "CDC45 is required for the loading of DNA replication origins in human cells"
**作者**: Pacek, M., et al.
**摘要**: 通过特异性CDC45抗体的免疫沉淀实验，研究CDC45在DNA复制起始复合体组装中的作用，证实其与MCM和GINS复合物的相互作用。
2. **文献名称**: "Dynamic regulation of CDC45 during the cell cycle and its role in DNA replication checkpoint control"
**作者**: Walter, D., et al.
**摘要**: 利用CDC45抗体进行Western blot和免疫荧光分析，揭示CDC45在S期检查点中的功能，及其与ATR激酶通路的关联。
3. **文献名称**: "Overexpression of CDC45 as a biomarker in colorectal cancer progression"
**作者**: Zhang, L., et al.
**摘要**: 通过免疫组化(使用CDC45特异性抗体)发现CDC45在结直肠癌组织中高表达，并与患者预后不良相关。
4. **文献名称**: "Characterization of a novel monoclonal antibody against human CDC45 for cancer research"
**作者**: Li, Y., et al.
**摘要**: 报道一种新型CDC45单克隆抗体的开发与验证，证实其在流式细胞术和免疫共沉淀中的高特异性，应用于多种癌症模型研究。
(注：以上文献为示例，实际引用需根据真实研究调整。)
The CDC45 antibody is a crucial tool in studying DNA replication and cell cycle regulation. CDC45 (Cell Division Cycle 45) is an essential protein required for the initiation and elongation phases of eukaryotic DNA replication. It forms part of the CMG (CDC45-MCM-GINS) helicase complex, which unwinds DNA at replication forks. This protein interacts with minichromosome maintenance (MCM) proteins and GINS components to activate replication origins during the G1/S transition.
Antibodies targeting CDC45 are widely used to investigate its expression dynamics, localization, and interactions during cell cycle progression. Researchers employ these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze CDC45's role in replication fork stability, checkpoint responses, and replication stress. Altered CDC45 expression or function has been linked to genomic instability and diseases, including cancer, making its detection valuable in oncology studies.
Commercial CDC45 antibodies are typically raised against specific epitopes in human or model organism variants (e.g., mouse, Xenopus), with validation in knockdown/knockout models ensuring specificity. Proper controls are critical due to potential cross-reactivity with related replication factors. Recent applications include single-molecule imaging studies and diagnostic exploration of replication-related disorders.
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