| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSPA14; Heat shock protein HSP60; Heat shock 70 kDa protein 14; HSP70L1; HSP60; Heat shock 70kDa protein 14; HSP70-like protein 1; HSP70-4;;Hsp70 14 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Hsp70 14 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及HSPA14抗体的模拟参考文献示例(注：部分内容为概括性示例，实际文献需通过数据库验证)：
1. **文献名称**: "HSPA14 interacts with BAG6 to regulate protein quality control and cancer cell apoptosis"
**作者**: Zhang Y, et al.
**摘要**: 研究揭示了HSPA14通过BAG6复合物参与错误折叠蛋白的降解通路，使用HSPA14抗体进行免疫共沉淀实验，证明其在结肠癌细胞中调控蛋白酶体依赖性凋亡的作用。
2. **文献名称**: "HSPA14 expression correlates with neuronal stress resistance in Alzheimer's disease models"
**作者**: Lee S, et al.
**摘要**: 通过HSPA14抗体进行脑组织免疫组化分析，发现HSPA14在阿尔茨海默病模型神经元中表达上调，可能通过抑制Tau蛋白异常磷酸化发挥神经保护作用。
3. **文献名称**: "A novel HSPA14 antibody-based assay for detecting viral infection-induced ER stress"
**作者**: Gupta R, et al.
**摘要**: 开发了一种高特异性HSPA14单克隆抗体，应用于流感病毒感染的细胞模型中，证实HSPA14在内质网应激反应中与IRE1α信号通路相互作用。
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**建议查询方式**：
- 使用PubMed/Google Scholar检索 "HSPA14 antibody" + "application" 或结合具体研究领域(如癌症、病毒感染)。
- 关注近年文献(2020年后)，部分研究可能涉及HSPA14在COVID-19等新兴领域的作用。
- 企业抗体说明书(如Cell Signaling Technology官网)也可能提供关键引用文献。
**Background of HSPA14 Antibody**
The HSPA14 (Heat Shock Protein Family A Member 14) antibody is a research tool designed to detect and study the HSPA14 protein, a member of the heat shock protein 70 (HSP70) family. HSP70 proteins are molecular chaperones critical for maintaining cellular homeostasis, assisting in protein folding, preventing aggregation, and facilitating stress response. HSPA14. also known as HSP70L1. is distinguished by its unique expression patterns and functional roles. It localizes to the endoplasmic reticulum (ER) and mitochondria, participating in protein quality control and stress adaptation.
HSPA14 is implicated in diverse physiological and pathological processes, including cancer progression, neurodegenerative diseases, and immune regulation. Its overexpression has been observed in certain cancers, where it may promote cell survival under stress. In neurodegenerative contexts, dysregulation of HSPA14 correlates with protein misfolding disorders.
Antibodies targeting HSPA14 are essential for investigating its expression, interaction partners, and mechanisms in disease models. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to validate protein levels, tissue distribution, and subcellular localization. Validated HSPA14 antibodies often undergo knockout/knockdown controls to ensure specificity.
Research utilizing HSPA14 antibodies contributes to understanding its role in cellular stress responses, potential therapeutic targeting, and biomarker discovery. As interest grows in HSP70 family members as drug targets, HSPA14 remains a focus for exploring novel interventions in cancer and neurodegeneration.
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