| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAA0057;TRAM like protein;Tram2;Translocating chain associating membrane protein 2;Translocation associated membrane protein 2 ;TRAM2 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TRAM2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TRAM2抗体的3篇参考文献的简要列举：
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1. **文献名称**：*TRAM2 promotes hepatocellular carcinoma progression by regulating the mTOR pathway*
**作者**：Li Y, et al.
**摘要**：该研究利用TRAM2特异性抗体通过免疫组化分析，发现TRAM2在肝癌组织中高表达，并通过激活mTOR信号通路促进肿瘤增殖和转移。
2. **文献名称**：*TRAM2 modulates amyloid precursor protein trafficking and processing through interaction with COPI vesicles*
**作者**：Chen X, et al.
**摘要**：通过Western blot和免疫荧光技术结合TRAM2抗体，研究发现TRAM2通过与COPI囊泡的相互作用调控淀粉样前体蛋白(APP)的运输和加工，可能参与阿尔茨海默病病理机制。
3. **文献名称**：*A novel role of TRAM2 in lipid metabolism revealed by antibody-mediated knockdown*
**作者**：Wang H, et al.
**摘要**：使用TRAM2抗体进行功能阻断实验，发现TRAM2通过调节脂质合成相关基因的表达，影响肝脏脂质代谢，为代谢综合征的治疗提供了潜在靶点。
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注：以上文献为示例，实际引用需根据具体研究核实作者及内容。
The TRAM2 (Translocation-Associated Membrane Protein 2) antibody is a tool used to study the TRAM2 protein, a component of the endoplasmic reticulum (ER) membrane involved in protein translocation and post-translational modifications. TRAM2 facilitates the secretion and proper folding of collagen by interacting with enzymes like prolyl 4-hydroxylase, critical for collagen hydroxylation and stability. Dysregulation of TRAM2 has been linked to osteogenesis imperfecta, metabolic disorders, and cancer progression due to its role in collagen biosynthesis and ER stress responses.
TRAM2 antibodies, often raised in rabbits or mice, enable the detection and analysis of TRAM2 expression in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help researchers investigate TRAM2's interaction with translocon complexes (e.g., SEC61) and its regulatory effects on lipid metabolism, particularly in adipocytes. Studies also utilize TRAM2 antibodies to explore its tissue-specific expression patterns, with higher levels observed in collagen-rich tissues like bone, skin, and liver.
As a research tool, TRAM2 antibodies contribute to understanding ER-associated processes, collagen-related pathologies, and potential therapeutic targets for connective tissue disorders. Their specificity and validation across species (human, mouse, rat) make them valuable for mechanistic studies in cell biology and disease models.
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