| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; KAP1; TF1B; RNF96; TIF1B; TRIM28;;TIF1 beta |
| WB Predicted band size | Calculated MW: 89 kDa ; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TIF1 beta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于KAP1(TRIM28)抗体的3篇参考文献示例，涵盖不同研究方向及实验应用：
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1. **文献名称**：*KAP1 controls endogenous retroviruses in embryonic stem cells*
**作者**：Rowe HM et al.
**摘要**：该研究揭示KAP1通过表观遗传沉默调控胚胎干细胞中的逆转录转座子。作者利用KAP1抗体进行ChIP-seq实验，证实KAP1与SETDB1组蛋白甲基转移酶协同作用，介导H3K9me3修饰，抑制逆转录病毒活性。
2. **文献名称**：*TRIM28 is essential for nuclear reprogramming and pluripotency*
**作者**：Messerschmidt DM et al.
**摘要**：研究证明KAP1(TRIM28)在多能性维持和重编程中的关键作用。通过Western blot和免疫荧光实验(使用KAP1抗体)，发现KAP1缺失导致胚胎干细胞分化异常，并影响Oct4/Sox2的转录调控网络。
3. **文献名称**：*Phosphorylation of KAP1 by ATM promotes the DNA damage response*
**作者**：White D et al.
**摘要**：该文阐明DNA损伤后，ATM激酶磷酸化KAP1的S824位点，促进染色质松弛和修复。研究利用特异性KAP1抗体(针对磷酸化修饰)进行免疫沉淀，揭示KAP1在调控同源重组修复中的动态修饰机制。
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以上文献展示了KAP1抗体在ChIP-seq、免疫荧光、Western blot及修饰位点检测中的广泛应用，涵盖表观遗传、干细胞及DNA修复领域。如需更多文献，可进一步限定研究主题或实验方法。
KAP1 (KRAB-associated protein 1), also known as TRIM28 or TIF1β, is a multifunctional transcriptional regulator critical for epigenetic silencing and chromatin remodeling. It serves as a co-repressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs), mediating gene silencing by recruiting histone-modifying enzymes like SETDB1 (a histone methyltransferase) and HP1 (heterochromatin protein 1) to establish heterochromatin. KAP1 plays pivotal roles in embryonic development, genomic imprinting, and retrotransposon suppression.
Structurally, KAP1 contains a RBCC motif (RING, B-box, coiled-coil) and a PHD-bromodomain, enabling interactions with chromatin and other proteins. Post-translational modifications, such as phosphorylation and SUMOylation, regulate its activity. For example, phosphorylation at Ser824 modulates its role in DNA damage response.
KAP1 antibodies are essential tools in studying these processes. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect KAP1 expression, localization, and interactions. In research, KAP1 dysregulation is linked to cancer, viral latency, and neurological disorders. Antibodies against specific epitopes or modified forms (e.g., phosphorylated KAP1) help dissect its context-dependent functions. Commercial KAP1 antibodies are typically validated for specificity across human, mouse, and rat samples, supporting diverse experimental models.
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