| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85; TRAF6; RNF85; TRAF 6; TRAF-6;;TRAF6 |
| WB Predicted band size | Calculated MW: 60 kDa ; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TRAF6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TRAF6抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*TRAF6 deficiency results in osteopetrosis and defective interleukin-1. CD40. and LPS signaling*
**作者**：Lomaga MA, et al.
**摘要**：该研究通过基因敲除小鼠模型，发现TRAF6在骨代谢和免疫信号通路(如IL-1、CD40和TLR/NF-κB通路)中起关键作用，实验中使用TRAF6抗体验证蛋白表达缺失及其对下游信号的影响。
2. **文献名称**：*TAK1 is a ubiquitin-dependent kinase of MKK and IKK*
**作者**：Wang C, et al.
**摘要**：文章揭示了TRAF6通过泛素化修饰激活TAK1-MKK/IKK信号轴，进而调控NF-κB和MAPK通路。实验中利用TRAF6抗体进行免疫共沉淀，证实其与TAK1复合物的相互作用。
3. **文献名称**：*TRAF6 drives IL-1β-induced NLRP3 inflammasome activation by promoting ubiquitination of ASC*
**作者**：Burns K, et al.
**摘要**：研究发现TRAF6通过介导ASC蛋白的泛素化，促进NLRP3炎症小体激活。实验通过TRAF6抗体敲低或抑制其功能，证明其在炎症反应中的关键调控作用。
4. **文献名称**：*TRAF6 function in B cells: implications in autoimmune diseases*
**作者**：Kingeter LM, Schaefer BC
**摘要**：该研究探讨TRAF6在B细胞中的特异性功能，表明其通过调控NF-κB信号影响自身免疫反应，实验中使用TRAF6抗体分析其在B细胞中的表达及定位。
这些文献涵盖了TRAF6在信号转导、炎症调控和疾病机制中的关键作用，并展示了抗体在蛋白检测、互作分析和功能研究中的应用。
TRAF6 (TNF receptor-associated factor 6) is a critical adaptor protein and E3 ubiquitin ligase involved in innate immune signaling, inflammation, and cellular homeostasis. It mediates signaling pathways downstream of Toll-like receptors (TLRs), interleukin-1 receptors (IL-1Rs), and the TNF receptor superfamily, facilitating activation of NF-κB and MAPK pathways. TRAF6 catalyzes K63-linked ubiquitination, a non-degradative post-translational modification essential for recruiting downstream signaling components. Structurally, it contains a RING domain enabling ubiquitin ligase activity and a TRAF domain for protein interactions.
TRAF6 antibodies are vital tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect TRAF6 in cell lysates, tissue samples, or signaling complexes. Specific monoclonal or polyclonal antibodies (produced in rabbits, mice, etc.) target distinct epitopes, enabling researchers to explore TRAF6's role in physiological processes (e.g., osteoclastogenesis, autophagy) and diseases such as cancer, autoimmune disorders, and neurodegenerative conditions. Validation via knockout controls or siRNA knockdown ensures antibody specificity. Dysregulation of TRAF6 is linked to chronic inflammation and tumorigenesis, making its antibodies crucial for both mechanistic research and therapeutic target validation.
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