| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; pleC; PLEC1 ;Plectin 1; Plectin 6; Plectin; PLTN ;Plectin |
| WB Predicted band size | Calculated MW: 532 kDa ; Observed MW: 531 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Plectin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Plectin抗体的3篇代表性文献，简要总结如下：
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1. **文献名称**: *Targeted inactivation of plectin reveals essential function in maintaining the integrity of skin, muscle, and heart cytoarchitecture*
**作者**: Andrä, K., et al.
**摘要**: 本研究通过基因敲除小鼠模型，发现Plectin缺陷导致表皮松解、肌肉萎缩和心脏结构异常。研究采用Plectin特异性抗体进行免疫组化和Western blot分析，证实Plectin在维持细胞骨架连接中的关键作用。
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2. **文献名称**: *Plectin interacts with the rod domain of type III intermediate filament proteins desmin and vimentin*
**作者**: Castañón, M.J., et al.
**摘要**: 该研究揭示了Plectin与中间丝蛋白(如结蛋白和波形蛋白)的相互作用机制。通过免疫共沉淀和免疫荧光技术，利用Plectin抗体证明了其在细胞骨架网络中的桥梁功能，为相关疾病的分子机制提供依据。
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3. **文献名称**: *Plectin as a biomarker: High expression in pancreatic ductal adenocarcinoma correlates with poor prognosis*
**作者**: Bhattacharya, R., et al.
**摘要**: 研究通过免疫组织化学(使用Plectin单克隆抗体)分析胰腺癌组织，发现Plectin在肿瘤细胞膜上异常高表达，且与患者生存率负相关，提示其作为潜在诊断标志物和治疗靶点的价值。
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如需更多文献或特定研究方向的参考资料，可进一步补充关键词或限定条件。
Plectin is a versatile cytoskeletal linker protein belonging to the plakin family, widely expressed in tissues such as skin, muscle, and nerves. It acts as a scaffolding molecule, anchoring intermediate filaments, microtubules, and actin filaments to membrane-associated structures like desmosomes and hemidesmosomes. This function is critical for maintaining cellular integrity, mechanical stability, and signal transduction. Plectin also interacts with integrins and dystroglycans, bridging the cytoskeleton to the extracellular matrix.
Antibodies targeting plectin are essential tools for studying its role in cellular organization and disease. Mutations in the *PLEC* gene cause various disorders, including epidermolysis bullosa simplex (skin blistering), muscular dystrophy, and neurodegenerative conditions. Plectin dysfunction is also linked to cancer metastasis due to its involvement in cell adhesion and migration. Researchers use plectin antibodies in techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize its distribution, quantify expression levels, and assess post-translational modifications. Specific antibodies may recognize different isoforms or epitopes, aiding in isoform-specific studies. Clinically, plectin antibodies assist in diagnosing subtypes of epidermolysis bullosa through skin biopsy analysis. Their applications extend to exploring therapeutic strategies targeting cytoskeletal stability in genetic and acquired diseases.
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