| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3 histone family, member A; H3/A; H31; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A;p-Histone H3.3 (T3) |
| WB Predicted band size | Calculated MW: 15 kDa ; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Histone H3.3 around the phosphorylation site of T3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-Histone H3 (T3)抗体的3篇参考文献(信息基于领域内常见研究方向整理，非真实文献)：
1. **文献名称**：*Phosphorylation of histone H3 at threonine 3 during mitosis and its role in chromosome segregation*
**作者**：Dai et al.
**摘要**：研究揭示了有丝分裂中Histone H3 T3磷酸化的动力学，表明其通过调控染色体凝缩和姐妹染色单体分离参与基因组稳定性维持，文中使用特异性抗体验证了该修饰的细胞周期依赖性表达。
2. **文献名称**：*Aurora kinase B phosphorylates histone H3 at threonine 3 to regulate mitotic progression*
**作者**：Thompson et al.
**摘要**：发现Aurora激酶B直接磷酸化Histone H3 T3位点，并通过该修饰调控纺锤体组装检查点功能。研究采用Phospho-H3(T3)抗体进行免疫荧光染色，证实其作为有丝分裂进程的生物标志物。
3. **文献名称**：*Phospho-histone H3 (T3) as a novel prognostic marker in glioblastoma*
**作者**：Chen et al.
**摘要**：探讨了Phospho-H3(T3)在胶质母细胞瘤中的表达与患者预后的相关性，利用该抗体的免疫组化分析显示其高表达与肿瘤增殖活性及不良生存率显著相关。
(注：以上文献为示例性质，实际引用时需查询真实数据库。)
The Phospho-Histone H3 (Thr3) antibody is a specialized tool used to detect histone H3 phosphorylated at threonine 3 (H3T3ph), a post-translational modification associated with chromatin dynamics during cell division. Histone H3. a core component of nucleosomes, undergoes phosphorylation at specific residues to regulate chromatin condensation and chromosome segregation in mitosis. While phosphorylation at serine 10 (S10) or serine 28 (S28) is more commonly linked to mitotic progression, Thr3 phosphorylation has been implicated in early mitotic events, such as the activation of the Aurora B kinase complex at centromeres. This modification may also play a role in epigenetic regulation by influencing interactions with chromatin-binding proteins.
The antibody is widely utilized in cell biology and cancer research to study mitotic activity, particularly in tissues or cultured cells. It serves as a marker for mitotic cells in techniques like immunofluorescence, flow cytometry, or immunohistochemistry, aiding in the assessment of proliferation rates in tumors or drug response studies. Specificity validation (e.g., peptide blocking assays) is critical, as cross-reactivity with other phospho-epitopes can occur. Research using this antibody has contributed to understanding cell cycle dysregulation in diseases, including cancer, where aberrant mitotic signaling is a hallmark. Its applications extend to developmental biology and neurobiology, exploring cell proliferation in tissue-specific contexts.
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