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Rabbit Monoclonal PPP1CA+1CB Antibody

  • 中文名： PPP1CA+1CB抗体
  • 别    名： PP-1A; PP-1B; PP1A; PP1alpha; PP1B; PP1beta; PPP1A; PPP1CD;PPP1A/B
货号: IPDX17055
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPP-1A; PP-1B; PP1A; PP1alpha; PP1B; PP1beta; PPP1A; PPP1CD;PPP1A/B
WB Predicted band sizeCalculated MW: 38,37 kDa ; Observed MW: 37 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PPP1A
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于PPP1CA和PPP1CB抗体的3篇参考文献示例(注：以下文献信息为虚构示例，仅供格式参考)：

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1. **文献名称**: "Differential Roles of PPP1CA and PPP1CB in Cellular Signaling Pathways"

**作者**: Zhang et al.

**摘要**: 本研究利用特异性PPP1CA和PPP1CB抗体，通过免疫印迹和免疫荧光技术，揭示了两种亚基在MAPK和AKT信号通路中的独特定位及功能差异，为靶向治疗提供依据。

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2. **文献名称**: "PPP1CB-Specific Antibody Reveals Its Role in Neuronal Differentiation"

**作者**: Thompson et al.

**摘要**: 通过开发高特异性PPP1CB抗体，研究发现该亚基通过调节CREB去磷酸化促进神经元分化，抗体验证实验包括敲除细胞系的Western blot分析。

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3. **文献名称**: "Validation of Commercial Antibodies for PPP1CA in Cancer Biomarker Studies"

**作者**: Gomez et al.

**摘要**: 评估了多种商业化PPP1CA抗体的特异性，结合质谱验证，发现部分抗体在乳腺癌组织中的交叉反应性，强调抗体选择对临床研究的重要性。

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**备注**：以上文献为示例，实际研究中请通过PubMed或Google Scholar检索真实文献(可用关键词：PPP1CA antibody, PPP1CB antibody, validation, signaling等)。若需具体文献，建议补充更详细的研究背景或应用场景。

背景信息

The PPP1CA and PPP1CB antibodies target catalytic subunits of protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for regulating diverse cellular processes. PP1 exists as a holoenzyme composed of a catalytic subunit (encoded by PPP1CA, PPP1CB, or PPP1CC genes) and regulatory interactors that dictate substrate specificity. PPP1CA (α isoform) and PPP1CB (β isoform) share ~90% sequence identity but exhibit tissue-specific expression and functional divergence. These isoforms participate in dephosphorylation events governing cell cycle progression, metabolic signaling, DNA damage repair, and neuronal signaling. Antibodies against PPP1CA/1CB enable isoform-specific detection, aiding studies on their spatial-temporal expression, subcellular localization, and disease associations. Due to high sequence homology, antibody specificity often relies on targeting variable regions or isoform-unique C-terminal residues. Applications include Western blotting, immunofluorescence, and co-immunoprecipitation to explore PP1 interactions with regulatory proteins (e.g., inhibitor-1. spinophilin) or substrates. Dysregulation of PP1 isoforms is implicated in cancer, Alzheimer’s disease, and cardiac pathologies, making these antibodies valuable tools for mechanistic studies and therapeutic target validation. Validation via knockout/knockdown controls is essential to confirm specificity, given potential cross-reactivity between isoforms.

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