| WB | 1/1000-1/2000 | Human,Cow |
| IF | 咨询技术 | Human,Cow |
| IHC | 1/100-1/200 | Human,Cow |
| ICC | 技术咨询 | Human,Cow |
| FCM | 咨询技术 | Human,Cow |
| Elisa | 咨询技术 | Human,Cow |
| Aliases | Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1;;Collagen I |
| WB Predicted band size | Calculated MW: 139,129 kDa ; Observed MW: 139 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Cow |
| Immunogen | A synthesized peptide derived from human Collagen I |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Collagen I抗体的参考文献及其摘要概括：
1. **"Collagen type I-specific monoclonal antibody: A tool for immunohistochemical localization in tissues"**
- *作者：Linsenmayer TF 等*
- 摘要：开发并验证了一种特异性识别Collagen I的单克隆抗体，通过免疫组化技术检测小鼠皮肤、骨骼等组织中的Collagen I分布，证实其在组织工程研究中的应用价值。
2. **"Comparative analysis of collagen I antibody specificity in fibrotic liver models"**
- *作者：Henderson NC 等*
- 摘要：比较了不同商业来源的Collagen I抗体在肝纤维化模型中的特异性，发现部分抗体存在交叉反应性，提出标准化抗体验证流程以提高实验可重复性。
3. **"Immunofluorescence localization of collagen I in osteogenesis imperfecta using chain-specific antibodies"**
- *作者：Bateman JF 等*
- 摘要：利用α1和α2链特异性抗体分析成骨不全症患者骨组织，揭示Collagen I链比例异常与疾病严重程度的相关性，为遗传性骨病诊断提供新方法。
4. **"Collagen I antibody-based detection of extracellular matrix remodeling in cancer progression"**
- *作者：Levental KR 等*
- 摘要：通过Collagen I抗体标记肿瘤微环境，证明基质中Collagen I的线性排列与乳腺癌转移风险增加相关，为癌症预后评估提供生物标志物。
(注：以上文献标题和作者为模拟内容，实际引用需核实具体文献信息。)
Collagen I antibody is a widely used tool in biomedical research and diagnostics, targeting the most abundant extracellular matrix protein in the human body. Collagen I, composed of two α1 chains and one α2 chain (α1[I]₂α2[I]), forms a triple-helical structure critical for providing tensile strength to tissues such as bone, skin, tendons, and fibrous cartilage. Its expression is closely associated with tissue development, repair, and fibrosis.
Collagen I antibodies are primarily employed to detect and quantify Collagen I expression in various experimental techniques, including immunohistochemistry (IHC), immunofluorescence (IF), Western blotting, and ELISA. These applications help researchers study Collagen I's role in pathological conditions like fibrosis (e.g., liver cirrhosis, pulmonary fibrosis), cancer progression (e.g., tumor stroma remodeling), and bone disorders (e.g., osteogenesis imperfecta). In diagnostics, Collagen I antibodies aid in assessing tissue remodeling in biopsies, particularly in fibrotic diseases or wound healing models.
Commercially available Collagen I antibodies are often raised against specific epitopes, such as the N-terminal or C-terminal regions, with common clones including COL-1 (Sigma-Aldrich) and polyclonal variants from rabbits or mice. Validation typically includes reactivity checks across species (human, mouse, rat) and specificity testing to avoid cross-reactivity with other collagen types (e.g., III, V). Recent advancements in regenerative medicine and 3D tissue engineering have further driven demand for reliable Collagen I antibodies to monitor matrix deposition in engineered tissues.
Overall, Collagen I antibodies remain indispensable for understanding extracellular matrix dynamics in health and disease.
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