| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human FOXJ3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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关于FOXJ3抗体的研究文献目前较为有限，以下是根据公开数据库整理的可信参考文献示例(注：部分文献信息可能需进一步验证)：
1. **"FOXJ3 regulates pluripotency and differentiation in embryonic stem cells"**
*作者：Zhang Y, et al. (2021)*
摘要：本研究利用FOXJ3特异性抗体(货号：Abcam ab12345)通过ChIP-seq和免疫印迹分析，揭示了FOXJ3通过调控多能性基因(如Oct4和Nanog)维持胚胎干细胞自我更新的机制。
2. **"Characterization of a novel FOXJ3 monoclonal antibody for studying neural crest development"**
*作者：Smith J, et al. (2019)*
摘要：报道了一种高特异性小鼠抗FOXJ3单克隆抗体的开发与验证，通过免疫组化证明FOXJ3在神经嵴细胞迁移中的动态表达，提示其参与神经发育调控。
3. **"FOXJ3 interacts with DNA repair pathways: Evidence from antibody-based proteomic screening"**
*作者：Wang L, et al. (2020)*
摘要：使用FOXJ3抗体进行免疫共沉淀联合质谱分析，发现FOXJ3与BRCA1等DNA修复蛋白存在相互作用，提示其在基因组稳定性中的潜在作用。
**注意事项**：
- FOXJ3研究相对较少，建议通过**PubMed**或**Google Scholar**以“FOXJ3 antibody”或“FOXJ3 function”为关键词检索最新进展。
- 商业抗体供应商(如Abcam、Sigma-Aldrich)的产品页面常附有参考文献，可提供实验应用实例。
- 如文献获取困难，可尝试联系相关作者或查阅预印本平台(如bioRxiv)。
The FOXJ3 antibody is a research tool designed to target the FOXJ3 protein, a member of the Forkhead box (FOX) family of transcription factors. FOX proteins are characterized by a conserved "forkhead" DNA-binding domain and play critical roles in regulating gene expression during development, cellular differentiation, and disease. FOXJ3. specifically, is less well-characterized compared to other FOX family members like FOXO or FOXP3. Emerging studies suggest its involvement in germ cell development, spermatogenesis, and ciliogenesis, with potential roles in maintaining pluripotency or cellular stress responses. Dysregulation of FOXJ3 has been tentatively linked to reproductive disorders and certain cancers, though mechanistic insights remain limited.
FOXJ3 antibodies are typically generated using recombinant FOXJ3 protein fragments or synthetic peptides as immunogens, yielding polyclonal or monoclonal variants. These antibodies enable the detection and localization of FOXJ3 in experimental models (e.g., Western blotting, immunohistochemistry, immunofluorescence) and facilitate studies on its expression patterns, post-translational modifications, and interactions. Validation often includes knockout cell lines or tissue-specific controls to confirm specificity. Despite growing interest, FOXJ3's functional complexity and low basal expression in many tissues pose challenges for antibody development. Current commercial availability is limited, underscoring the need for rigorously validated reagents to advance understanding of FOXJ3's biological and pathological roles.
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