首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人类白细胞抗原B27(HLA-B27)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、脑脊液（CSF）样本中的HLA-B27含量。HLA-B27是人类白细胞抗原B27。它与脊柱关节病尤其是强直性脊柱炎密切相关。研究机制上，HLA-B27可能通过分子模拟、关节源性肽及免疫调节异常等机制，引发机体异常免疫反应，导致关节等部位的炎症与病变。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可为免疫性疾病机制研究、炎症反应通路探索以及HLA - B27表达水平与疾病相关性分析提供可靠工具，尤其适用于神经免疫、风湿病学等领域的体液样本检测需求，其多样本类型的兼容性为跨组织液比较研究创造了便利条件。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HLA-B27
Uniprot No.：P03989
种属：Homo sapiens (Human)
样本类型：serum, urine, cerebrospinal fluid (CSF)
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-96 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-110 | |||||
回收率：
| The recovery of human HLA-B27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.7302.6122.6712.563 102.3612.2412.3012.193 51.7021.6381.6701.562 2.50.9330.8710.9020.794 1.250.5340.5220.5280.420 0.6250.3240.3180.3210.213 0.3120.2430.2360.2400.132 00.1090.1070.108 | ||||||
货期：3-5 working days
×
