首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人富组蛋白(histatin-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、唾液样本中的histatin-5含量。Histatin-5是一种由唾液腺分泌的低分子量蛋白质，具有抗菌和抗真菌活性。其研究机制主要涉及抑制细菌和真菌的细胞膜完整性，干扰其代谢和复制过程，以及调节宿主的免疫反应。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于研究口腔微生物感染、炎症反应调控或免疫调节机制等科研领域；可用于评估口腔疾病模型、抗菌肽功能研究或唾液成分分析，为探索口腔黏膜免疫及宿主 - 微生物互作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：histatin-5
种属：Homo sapiens (Human)
样本类型：serum, plasma, saliva
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Histatin 5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of human Histatin 5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.4482.4542.4512.2604002.0242.1672.0961.9052001.6291.6781.6541.4631001.1511.1691.1600.969500.6220.6340.6280.437250.3750.3910.3830.19212.50.3120.3240.3180.12700.1920.1900.191 | ||||||
货期：3-5 working days
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