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产品描述：人组蛋白H4(HIST1H4A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HIST1H4A含量。HIST1H4A 是重要的组蛋白编码基因，参与染色体结构和基因表达调控。在研究机制上，其表达异常与细胞增殖、分化及凋亡等过程相关，还可能与一些疾病发生发展存在联系，或可成为疾病诊断和治疗潜在靶点。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，本试剂盒适用于体外科研场景中蛋白质相互作用研究、表观遗传学机制探索以及细胞分化/凋亡相关模型的分子水平分析，其宽泛的检测范围和多样本兼容性为科研人员提供了灵活的实验方案选择，可满足基础医学研究、分子生物学实验等多种需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：HIST1H4A
Uniprot No.：P62805
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.3 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 101 | ||||
| Range % | 95-108 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 83-99 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 87-92 | |||||
| 1:8 | Average % | 110 | ||||
| Range % | 106-115 | |||||
回收率：
| The recovery of human HIST1H4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 24002.7572.6572.7072.539 12002.0241.9241.9741.806 6001.2261.1461.1861.018 3000.6650.6550.6600.492 1500.3900.3800.3850.217 750.2810.2710.2760.108 37.50.2250.2150.2200.052 00.1730.1630.168 | ||||||
货期：3-5 working days
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