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产品描述:人谷氨酸谷草转氨酶2,线粒体(谷草转氨酶2)(GOT2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的GOT2含量。GOT2即谷草转氨酶 2,是一种参与细胞代谢的关键酶。其背景上,与多种生理过程尤其是氨基酸代谢相关。研究机制方面,它在细胞能量代谢、肿瘤发生发展等进程中发挥重要作用,影响肿瘤细胞增殖和转移,是潜在的疾病治疗靶点。试剂盒检测范围为0.156 mU/mL-10 mU/mL,可为科研人员探究肝脏疾病模型、代谢通路调控、药物作用机制等基础研究提供可靠工具,尤其适用于动物实验或体外培养细胞中GOT2活性动态变化的定量分析,为揭示线粒体相关分子机制提供技术支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:AATM_HUMAN ELISA Kit; AL022787 ELISA Kit; Aspartate aminotransferase 2 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase; mitochondrial ELISA Kit; Aspartate transaminase 2 ELISA Kit; ASPATA ELISA Kit; EC 2.6.1.1 ELISA Kit; FABP 1 ELISA Kit; FABP pm ELISA Kit; FABP-1 ELISA Kit; FABPpm ELISA Kit; Fatty acid binding protein ELISA Kit; Fatty acid-binding protein ELISA Kit; FLJ40994 ELISA Kit; Glutamate oxaloacetate transaminase 2 ELISA Kit; Glutamate oxaloacetate transaminase 2; mitochondrial ELISA Kit; Glutamate oxaloacetate transaminase; mitochondrial ELISA Kit; Glutamic oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2) ELISA Kit; Got 2 ELISA Kit; GOT2 ELISA Kit; KAT4 ELISA Kit; KATIV ELISA Kit; kynurenine aminotransferase 4 ELISA Kit; Kynurenine aminotransferase IV ELISA Kit; kynurenine--oxoglutarate transaminase 4 ELISA Kit; kynurenine--oxoglutarate transaminase IV ELISA Kit; mAspAT ELISA Kit; MGC102129 ELISA Kit; MGC115763 ELISA Kit; mitAAT ELISA Kit; mitochondrial ELISA Kit; Mitochondrial aspartate aminotransferase ELISA Kit; OTTMUSP00000017748 ELISA Kit; Plasma membrane fatty acid binding protein ELISA Kit; Plasma membrane-associated fatty acid-binding protein ELISA Kit; Transaminase A ELISA Kit
缩写:GOT2
Uniprot No.:P00505
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.156 mU/mL-10 mU/mL
灵敏度:0.039 mU/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GOT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 85 | |||||
Range % | 80-89 | ||||||
1:2 | Average % | 92 | |||||
Range % | 88-97 | ||||||
1:4 | Average % | 96 | |||||
Range % | 91-100 | ||||||
1:8 | Average % | 87 | |||||
Range % | 82-91 |
回收率:
The recovery of human GOT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 103 | 98-109 | |||||
EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
mU/mlOD1OD2AverageCorrected102.1862.2682.2272.02851.7651.7941.7801.5812.51.3911.3801.3861.1871.250.9280.9110.9200.7210.6250.6540.6230.6390.4400.3120.4390.4590.4490.2500.1560.3270.3410.3340.13500.2020.1950.199 |
货期:3-5 working days
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