首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人高尔基蛋白73(GP-73)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GOLM1含量。试剂盒检测范围为0.156 IU/mL-10 IU/mL，可广泛用于肝病模型构建、药物干预效果评估、细胞分泌功能分析及组织病理机制探索等领域，为肝脏相关疾病的基础研究提供可靠工具。支持科研人员高效完成高通量样本分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：bA379P1.3 ELISA Kit; C9orf155 ELISA Kit; Chromosome 9 open reading frame 155 ELISA Kit; Golgi membrane protein 1 ELISA Kit; Golgi membrane protein GP73 ELISA Kit; Golgi phosphoprotein 2 ELISA Kit; Golgi protein 73 kD ELISA Kit; Golgi protein 73kD ELISA Kit; GOLM 1 ELISA Kit; GOLM1 ELISA Kit; GOLM1_HUMAN ELISA Kit; GOLPH 2 ELISA Kit; GOLPH2 ELISA Kit; GP 73 ELISA Kit; GP73 ELISA Kit; PSEC0257 ELISA Kit
缩写：GOLM1
Uniprot No.：Q8NBJ4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 IU/mL-10 IU/mL
灵敏度：0.039 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GP-73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human GP-73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected102.0992.0932.0962.02551.4711.3711.4211.3502.50.9210.9010.9110.8401.250.5930.5550.5740.5030.6250.3940.3880.3910.3200.3120.2470.2350.2410.1700.1560.1510.1390.1450.07400.0730.0690.071 | ||||||
货期：3-5 working days
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