首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的GOT1含量。GOT1是一种重要靶点。它参与细胞内氨基酸代谢，在细胞能量生成和生物合成中起关键作用。研究发现其异常表达与多种疾病尤其是肿瘤发生发展相关，解析其相关机制可助力开发以GOT1为靶点的新型治疗药物和策略。试剂盒检测范围为3.12 mIU/mL-200 mIU/mL，在肝脏病理机制研究、心肌功能实验及药物毒性评估等科研场景中具有重要应用价值；适用于体外实验中的组织损伤模型评估、生化指标动态监测及相关分子机制探索，为疾病模型构建、药效学评价等基础研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Got1Aspartate aminotransferase ELISA kit; cytoplasmic ELISA kit; cAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.3 ELISA kit; Cysteine aminotransferase ELISA kit; cytoplasmic ELISA kit; Cysteine transaminase ELISA kit; cytoplasmic ELISA kit; cCAT ELISA kit; Glutamate oxaloacetate transaminase 1 ELISA kit; Transaminase A ELISA kit
缩写：GOT1
Uniprot No.：P13221
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：3.12 mIU/mL-200 mIU/mL
灵敏度：0.78 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-92 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected2002.0942.0682.0811.9181001.2761.3101.2931.130500.8380.7840.8110.648250.5210.5390.5300.36712.50.3530.3340.3440.1816.250.3000.2880.2940.1313.120.2410.2370.2390.07600.1640.1620.163 | ||||||
货期：3-5 working days
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