首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠OX-2膜糖蛋白(CD200)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD200含量。CD200是一种重要靶点。其作为跨膜糖蛋白，广泛表达于多种细胞表面。研究机制方面，CD200可与CD200R结合，调节免疫反应，在肿瘤免疫逃逸、自身免疫病等方面有重要作用，目前是免疫治疗等领域的研究热点。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于科研领域中对免疫调节机制的基础研究，如炎症性疾病模型、神经退行性疾病或肿瘤微环境中的免疫逃逸机制探索，也可用于评估实验动物模型中CD200表达水平与特定病理过程的相关性。适用于实验室常规检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd200; Mox2; OX-2 membrane glycoprotein; MRC OX-2 antigen; CD antigen CD200
缩写：CD200
Uniprot No.：P04218
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 106 | 102-113 | |||||
| EDTA plasma (n=4) | 98 | 94-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.2712.3172.2942.10315001.4291.3691.3991.2087500.9280.9840.9560.7653750.5890.5680.5790.388187.50.3960.3720.3840.193940.2880.3040.2960.105470.2400.2310.2360.04500.1880.1930.191 | |||||||
货期：3-5 working days
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