| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Soh1; CGI-125; 3110004H13Rik |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MED31 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及MED31抗体的参考文献及其摘要概述：
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1. **文献名称**：*MED31 regulates lipid metabolism and tumor progression in prostate cancer through transcriptional control of the PPARγ signaling pathway*
**作者**：Li et al.
**摘要**：该研究利用MED31抗体进行Western Blot和免疫组化实验，发现MED31通过调控PPARγ通路影响前列腺癌细胞的脂代谢，并与肿瘤转移相关。抗体用于检测MED31在癌组织中的表达水平及亚细胞定位。
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2. **文献名称**：*Role of MED31 in adipocyte differentiation and lipid storage*
**作者**：Zhang et al.
**摘要**：通过MED31抗体的ChIP-seq和免疫荧光技术，作者揭示MED31与转录因子CEBPα协同作用，调控脂肪生成相关基因的转录，影响脂肪细胞分化和脂滴形成。
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3. **文献名称**：*Developmental regulation of Mediator complex subunit MED31 in mammalian embryogenesis*
**作者**：Smith et al.
**摘要**：研究使用MED31抗体进行小鼠胚胎组织的免疫组化分析，发现MED31在早期胚胎发育中高表达，尤其在神经管和肢芽中，提示其参与器官发生和基因时空特异性表达调控。
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4. **文献名称**：*Structural insights into the Mediator complex: MED31 interaction dynamics*
**作者**：Kuroda et al.
**摘要**：通过免疫共沉淀结合质谱技术(使用MED31抗体)，解析MED31在中介体复合物中的构象变化及其对RNA聚合酶II招募的影响，揭示其动态互作机制。
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以上研究均明确提及MED31抗体的实验应用，涵盖癌症、代谢、发育及结构机制等领域。
The MED31 antibody targets the Mediator Complex Subunit 31 (MED31), a component of the multi-protein Mediator complex critical for regulating RNA polymerase II-driven transcription in eukaryotes. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED31. a conserved subunit, plays roles in stabilizing the complex and modulating specific transcriptional programs, particularly those linked to metabolic regulation, stress responses, and developmental pathways.
MED31 antibodies are widely used in research to investigate gene expression mechanisms, protein-protein interactions, and MED31's involvement in diseases. For example, studies have implicated MED31 in cancers, metabolic disorders, and neurodegenerative conditions, where its dysregulation may alter transcriptional outputs. These antibodies enable techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP) to assess MED31 expression, localization, and binding partners.
Developed in hosts such as rabbits or mice, MED31 antibodies are validated for specificity and affinity, often through knockout controls or siRNA knockdown. Their application has advanced understanding of MED31's structural contributions to the Mediator complex and its functional interplay with nuclear receptors or other transcription factors. Ongoing research leverages these tools to explore therapeutic targeting of Mediator subunits in disease contexts.
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