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产品描述：人CD177抗原(CD177)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的CD177含量。CD177是一种重要靶点，主要表达于中性粒细胞，在调节细胞黏附、迁移等方面发挥作用。研究发现它参与免疫反应与炎症过程，还和某些肿瘤的发展相关，科研人员正深入研究其信号通路机制，有望为相关疾病治疗提供新方向。试剂盒检测范围为0.9 ng/mL-60 ng/mL，该产品适用于炎症机制研究、免疫细胞功能分析以及疾病模型构建等科研领域，为探索CD177在感染、自身免疫性疾病或癌症微环境中的作用提供可靠工具，实验数据可支持细胞因子网络调控、药物靶点筛选等基础研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD177 ELISA Kit; CD177 antigen ELISA Kit; CD177 molecule ELISA Kit; CD177_HUMAN ELISA Kit; HNA-2a ELISA Kit; HNA2A ELISA Kit; Human neutrophil alloantigen 2a ELISA Kit; NB1 ELISA Kit; NB1 glycoprotein ELISA Kit; NB1 GP ELISA Kit; Polycythemia rubra vera 1 ELISA Kit; Polycythemia rubra vera protein 1 ELISA Kit; PRV-1 ELISA Kit; PRV1 ELISA Kit
缩写：CD177
Uniprot No.：Q8N6Q3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD177 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 95-108 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human CD177 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-100 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.6352.8632.7492.611301.8351.9951.9151.777151.1581.2561.2071.0697.50.5500.5330.5420.4043.750.3210.3410.3310.1931.80.2510.2490.2500.1120.90.2000.2060.2030.06500.1390.1370.138 | ||||||
货期：3-5 working days
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