首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠可溶性CD14(sCD14)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD14含量。CD14为一种细胞表面糖蛋白，是天然免疫中重要模式识别受体。它能识别细菌脂多糖等病原体相关分子模式。研究机制方面，可通过与配体结合，激活下游信号通路，促使细胞产生炎性因子等，在抗感染免疫等方面有重要作用。试剂盒检测范围为28 ng/mL-1800 ng/mL，常被用于研究感染性疾病和炎症相关机制；适用于研究感染模型、炎症性疾病或免疫调控过程中 sCD14 的动态变化，为探索宿主免疫应答机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd14Monocyte differentiation antigen CD14 ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit; CD antigen CD14 ELISA Kit
缩写：CD14
Uniprot No.：Q63691
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：28 ng/mL-1800 ng/mL
灵敏度：7 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 86-100 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-92 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of rat sCD14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 94-108 | ||||
| EDTA plasma (n=4) | 96 | 89-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected18002.5302.4012.4662.2839002.0661.9862.0261.8434501.3111.3561.3341.1512250.7590.7790.7690.586112.50.4560.4780.4670.284560.3520.3440.3480.165280.2710.2630.2670.08400.1810.1850.183 | ||||||
货期：3-5 working days
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