| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RRP2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human RHBDL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于RHBDL2抗体的参考文献及其简要概括：
1. **文献名称**：*RHBDL2 promotes cell motility by activating the Wnt-PCP pathway in breast cancer*
**作者**：Li X et al.
**摘要**：研究利用RHBDL2特异性抗体证实其在乳腺癌细胞中通过切割Wnt配体激活平面细胞极性通路，促进细胞迁移和侵袭。
2. **文献名称**：*Proteolytic activation of Notch by Rhomboid protease RHBDL2 in the Golgi apparatus*
**作者**：Urban S et al.
**摘要**：通过RHBDL2抗体定位实验，发现该蛋白酶在高尔基体中切割Notch受体，调控非经典Notch信号通路，影响细胞分化。
3. **文献名称**：*Characterization of RHBDL2-specific monoclonal antibodies for functional studies*
**作者**：Zhang Y et al.
**摘要**：开发并验证了针对RHBDL2的单克隆抗体，用于免疫印迹和免疫荧光，证明RHBDL2在多种癌细胞系中高表达且与基质金属蛋白酶活性相关。
(注：以上文献信息为模拟示例，实际引用请通过PubMed或Sci-Hub核实具体文献。)
The RHBDL2 (Rhomboid-like protein 2) antibody is a tool used to study the function and expression of the RHBDL2 protease, a member of the rhomboid family of intramembrane serine proteases. RHBDL2 is evolutionarily conserved and plays a role in cleaving transmembrane protein substrates, regulating processes like cell signaling, adhesion, and protein trafficking. Unlike other rhomboids, RHBDL2 is primarily localized to the plasma membrane and Golgi apparatus, where it processes substrates such as epidermal growth factor (EGF)-like ligands and adhesion G protein-coupled receptors (aGPCRs). Its activity is implicated in wound healing, angiogenesis, and cancer progression, particularly through modulation of EGFR and Notch signaling pathways.
Antibodies targeting RHBDL2 enable researchers to detect its expression levels, subcellular localization, and interactions in various tissues and cell lines. These reagents are critical for elucidating RHBDL2's physiological and pathological roles, including its potential as a biomarker or therapeutic target in diseases like colorectal cancer, where its overexpression correlates with tumor aggressiveness. Commercial RHBDL2 antibodies are typically validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence. However, challenges remain in ensuring specificity due to structural similarities among rhomboid family members. Ongoing research aims to clarify RHBDL2's substrate repertoire and regulatory mechanisms, leveraging these antibodies to advance understanding of intramembrane proteolysis in health and disease.
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