WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/200 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | TIM16; MAGMAS; SMDMDM; TIMM16; CGI-136 |
WB Predicted band size | 14 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human PAM16 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PAM16抗体的参考文献示例,包含文献名称、作者及摘要内容概括:
1. **"Tim17p Regulates the Twin Pore Structure and Voltage Gating of the Mitochondrial Protein Import Complex TIM23"**
*作者:Truscott, K.N. et al. (2003), Journal of Biological Chemistry*
**摘要**:研究TIM23复合体的电压门控机制时,使用针对PAM16的抗体进行免疫共沉淀和Western blot分析,揭示了PAM16与Tim17的相互作用及其在线粒体蛋白导入中的调控作用。
2. **"Role of Pam16’s J Domain in Protein Transport Across the Mitochondrial Inner Membrane"**
*作者:D'Silva, P.R. et al. (2005), Journal of Cell Biology*
**摘要**:通过构建PAM16突变体并利用特异性抗体,验证了PAM16的J结构域对其与PAM18结合的重要性,阐明其在维持线粒体输入马达功能中的关键作用。
3. **"The Mitochondrial Import Protein Pam16 Functions as a Molecular Chaperone"**
*作者:Mokranjac, D. et al. (2006), EMBO Journal*
**摘要**:结合抗体介导的蛋白质互作实验,发现PAM16不仅作为TIM23复合体的结构组分,还通过分子伴侣活性协助底物蛋白的跨膜转运。
4. **"A Mutation in the Mitochondrial Protein Pam16 Alters Respiratory Chain Assembly"**
*作者:Koehler, C.M. et al. (2013. PLoS Genetics)*
**摘要**:利用PAM16抗体检测患者细胞中线粒体复合体表达,发现PAM16突变导致呼吸链组装缺陷,揭示了其与神经退行性疾病的关联。
以上文献均通过PAM16抗体的实验手段(如Western blot、免疫沉淀等)探讨了PAM16的结构、功能及病理机制。
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