首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD109抗原(CD109)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD109含量。CD109是一种细胞表面糖蛋白，在多种组织细胞中表达。它与肿瘤发生、免疫调节等密切相关。研究机制方面，其可能通过参与信号传导通路影响细胞增殖、迁移等。目前围绕CD109作为靶点的药物研发等相关研究正在持续推进。试剂盒检测范围为94 pg/mL-6000 pg/mL，适用于基础科研领域，如探究CD109在肿瘤发生、免疫调控或组织修复中的分子机制，支持通过多种生物样本分析其在疾病模型或细胞实验中的动态表达变化，为体外实验提供高灵敏度的定量检测方案，本产品仅用于科学研究用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：150 KDa TGF beta 1 binding protein ELISA Kit; 150 KDa TGF-beta-1-binding protein ELISA Kit; 7D1 ELISA Kit; 8A3 ELISA Kit; Activated T cell marker CD109 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 ELISA Kit; CD 109 ELISA Kit; CD109 ELISA Kit; CD109 antigen ELISA Kit; CD109_HUMAN ELISA Kit; CPAMD7 ELISA Kit; DKFZp762L1111 ELISA Kit; E123 ELISA Kit; FLJ38569 ELISA Kit; FLJ41966 ELISA Kit; Gov system alloantigens on platelets ELISA Kit; p180 ELISA Kit; Platelet specific Gov antigen ELISA Kit; Platelet-specific Gov antigen ELISA Kit; r150 ELISA Kit; RP11 525G3.1 ELISA Kit
缩写：CD109
Uniprot No.：Q6YHK3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：94 pg/mL-6000 pg/mL
灵敏度：23.4 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD109 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of human CD109 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 85 | 81-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.0082.1572.0831.92830001.6041.6301.6171.46215001.1911.1751.1831.0287500.7840.7720.7780.6233750.4720.4980.4850.330187.50.3040.3150.3100.155940.2120.2160.2140.05900.1520.1570.155 | ||||||
货期：3-5 working days
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