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产品描述：人T复合物蛋白1亚基ζ(CCT6A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CCT6A含量。CCT6A是一种分子伴侣蛋白，属于CCT复合物成员，参与蛋白质折叠。研究发现，CCT6A在多种肿瘤中促进肿瘤生长和转移，其研究机制涉及调控细胞周期、增殖、侵袭和免疫抑制等过程。试剂盒检测范围为25 pg/mL-1600 pg/mL，科研人员可将其用于探究CCT6A在肿瘤发生、神经退行性疾病或代谢紊乱中的分子机制，也可用于评估细胞应激条件下伴侣蛋白系统的功能变化，或筛选影响蛋白质折叠通路的小分子化合物，为疾病基础研究及药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCT6A ELISA Kit; CCT6 ELISA Kit; CCTZ ELISA Kit; T-complex protein 1 subunit zeta ELISA Kit; TCP-1-zeta ELISA Kit; Acute morphine dependence-related protein 2 ELISA Kit; CCT-zeta-1 ELISA Kit; HTR3 ELISA Kit; Tcp20 ELISA Kit
缩写：CCT6A
Uniprot No.：P40227
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 83-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human CCT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-103 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.5612.4452.5032.3718002.1152.0172.0661.9344001.3821.4241.4031.2712000.7410.7360.7390.6071000.4220.4140.4180.286500.2640.2550.2600.128250.1910.1970.1940.06200.1330.1310.132 | ||||||
货期：3-5 working days
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