首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Apelin酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的APLN含量。APLN即Apelin基因编码的蛋白，Apelin-APJ信号通路参与心血管、代谢、神经等系统生理病理过程。其研究机制围绕调节血管张力、心脏功能，参与能量代谢平衡，在心血管疾病、肥胖等方面有潜在应用价值。试剂盒检测范围为125 pg/mL-8000 pg/mL，支持多样本类型检测，可广泛应用于心血管疾病模型研究、脂肪代谢调控实验或药物干预后Apelin分泌水平变化的体外机制探索，为生物医学领域研究提供稳定可靠的定量检测工具，实验结果仅用于科研目的。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AGTRL1 ligand ELISA Kit; APEL ELISA Kit; APEL_HUMAN ELISA Kit; Apelin 13 ELISA Kit; Apelin-13 ELISA Kit; APJ endogenous ligand ELISA Kit; Apln ELISA Kit; XNPEP2 ELISA Kit
缩写：APLN
Uniprot No.：Q9ULZ1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human apelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of human apelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 99 | 94-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.5322.4322.4822.35440001.9041.8041.8541.72620001.2871.1871.2371.10910000.7260.7160.7210.5935000.4570.4470.4520.3242500.3550.3450.3500.2221250.2370.2270.2320.10400.1290.1270.128 | ||||||
货期：3-5 working days
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