首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管紧张素原(aGT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液,组织培养上清液样本中的AGT含量。AGT即血管紧张素原，是肾素-血管紧张素系统（RAS）的唯一底物。其背景在于RAS与血压调节、水电解质平衡密切相关。研究机制主要是在肾素作用下AGT被水解成血管紧张素I，进一步转化成血管紧张素II，发挥收缩血管、升高血压等作用。试剂盒检测范围为78 pg/mL-5000 pg/mL，本试剂盒适用于基础科研中对 aGT 表达水平的动态监测，例如心血管疾病机制研究、药物干预效果评估或病理状态下体液标志物分析，尤其适合需要跨样本类型（如体液与细胞模型）对比的实验设计，为探索 RAS 系统调控及相关疾病靶点提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aangiotensinogen (serpin peptidase inhibitor clade A member 8) ELISA Kit; AGT ELISA Kit; AI265500 ELISA Kit; Alpha 1 antiproteinase antitrypsin ELISA Kit; Ang ELISA Kit; Ang I ELISA Kit; Ang II ELISA Kit; Ang III ELISA Kit; AngII ELISA Kit; Angiotensin I ELISA Kit; Angiotensin II ELISA Kit; Angiotensin III ELISA Kit; Angiotensin-3 ELISA Kit; Angiotensinogen (PAT) ELISA Kit; Angiotensinogen ELISA Kit; ANGT_HUMAN ELISA Kit; ANHU ELISA Kit; ANRT ELISA Kit; AT-2 ELISA Kit; AT-II ELISA Kit; Des-Asp[1]-angiotensin II ELISA Kit; FLJ92595 ELISA Kit; FLJ97926 ELISA Kit; MGC105326 ELISA Kit; PAT ELISA Kit; Pre angiotensinogen ELISA Kit; Serine (or cysteine) proteinase inhibitor ELISA Kit; Serpin A8 ELISA Kit; Serpin peptidase inhibitor clade A member 8 ELISA Kit; SERPINA8 ELISA Kit
缩写：AGT
Uniprot No.：P01019
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine,cell culture supernates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 85-100 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 93-110 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of human aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-100 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.5332.5892.5612.46725002.2342.2682.2512.15712501.6181.6281.6231.5296251.0051.0141.0100.9163120.4230.4530.4380.3441560.2360.2430.2400.146780.1490.1560.1530.05900.0910.0960.094 | ||||||
货期：3-5 working days
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