首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠刺鼠相关蛋白(AGRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGRP含量。AGRP即刺鼠相关蛋白，是一种与能量平衡调节密切相关的靶点。在研究机制方面，它主要通过作用于下丘脑弓状核，拮抗促黑素皮质素受体，抑制饱腹感，增加食欲和降低能量消耗。深入研究它可为肥胖等代谢疾病治疗提供新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于能量代谢调控、神经内分泌研究、肥胖相关动物模型评估以及药物干预实验中的AGRP动态监测，为探索摄食行为、能量稳态及相关疾病机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Agrp ELISA kit; Agrt ELISA kit; ArtAgouti-related protein ELISA kit
缩写：AGRP
Uniprot No.：P56473
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of mouse AGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5942.5682.5812.4045001.9251.8991.9121.7352501.2131.1781.1961.0191250.8070.7890.7980.62162.50.4540.4240.4390.26231.20.3170.3020.3100.13315.60.2640.2540.2590.08200.1780.1760.177 | ||||||
货期：3-5 working days
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