Mouse advanced glycation end products,AGEs ELISA Kit

大包装询价

货号: IPD-E09414m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠晚期糖基化终末产物(AGEs)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGEs含量。AGEs是晚期糖基化终末产物。背景上，它是糖与蛋白质等非酶促反应的产物，在糖尿病、衰老等过程中积累。研究机制表明，其通过与受体结合，激活多条信号通路，引发氧化应激、炎症反应等，参与糖尿病并发症等多种疾病发生发展。试剂盒检测范围为0.156 μg/mL-10 μg/mL，科研人员可借助探究AGEs在代谢性疾病模型中的动态变化、评估抗氧化或抗糖化药物的干预效果，以及衰老相关分子机制研究，为生物医学领域提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
AGEs
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.156 μg/mL-10 μg/mL
灵敏度：
0.039 μg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	97
	Range %	92-103
1:4	Average %	99
	Range %	94-110
1:8	Average %	95
	Range %	87-100

回收率：

The recovery of mouse AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-99
EDTA plasma (n=4)	97	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














µg/ml	OD1	OD2	Average	Corrected
10	1.958	1.900	1.929	1.779
5	1.583	1.602	1.593	1.443
2.5	1.160	1.154	1.157	1.007
1.25	0.754	0.739	0.747	0.597
0.625	0.471	0.434	0.453	0.303
0.312	0.342	0.319	0.331	0.181
0.156	0.253	0.239	0.246	0.096
0	0.152	0.148	0.150

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.