首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠聚集蛋白(AGRN)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGRN含量。AGRN 是一个靶点。它在人体生理系统中有特定功能。目前相关研究聚焦其在疾病发生发展中的作用机制，探索它如何参与信号传导、细胞间相互作用等过程，有望为开发针对 AGRN 的治疗手段，如药物研发，提供理论依据。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可将其应用于神经生物学、肌肉发育及再生机制研究，例如评估基因编辑模型小鼠中AGRN的表达水平变化，或探究疾病模型中细胞外基质蛋白动态调控过程，为神经肌肉系统相关的基础研究提供可靠工具。支持实验室常规的蛋白定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
缩写：AGRN
Uniprot No.：A2ASQ1
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of mouse AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-93 | ||||
| EDTA plasma (n=4) | 98 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.2572.1662.2122.038101.3611.3781.3701.19650.8580.8840.8710.6972.50.5720.5780.5750.4011.250.4470.4330.4400.2660.6250.3390.3540.3470.1730.3120.2480.2320.2400.06600.1760.1720.174 | ||||||
货期：3-5 working days
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