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产品描述：大鼠晚期糖基化终末产物(AGEs)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGEs含量。AGEs即晚期糖基化终末产物，在高血糖等情况下形成并不断累积。其研究机制主要是AGEs与受体结合后激活多条信号通路，引发氧化应激、炎症反应等，与糖尿病并发症、神经退行性疾病等多种疾病的发生发展密切相关。试剂盒检测范围为3.9 μg/mL-250 μg/mL，适用于糖尿病动物模型研究、衰老相关机制探索、氧化应激水平评估等科研领域，尤其适合在心血管疾病、神经退行性疾病等慢性病理模型的建立与验证中，通过血清、血浆或组织样本的动态监测揭示AGEs的生物学作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AGEs
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 μg/mL-250 μg/mL
灵敏度：0.97 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 86 | ||||
| Range % | 84-95 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:20 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 89-96 | |||||
回收率：
| The recovery of rat AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected2502.1942.2862.2402.0841251.7641.7891.7771.62162.51.2701.2561.2631.10731.20.9210.9750.9480.79215.60.4550.4650.4600.3047.80.2970.3080.3030.1473.90.2480.2330.2410.08500.1610.1500.156 | ||||||
货期：3-5 working days
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