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产品描述：人前梯度蛋白3同源物(AGR3)酶联免疫试剂盒,人前梯度蛋白3同源物(AGR3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AGR3含量。AGR3是一种与促癌基因AGR2高度相关的同源蛋白，属于蛋白质二硫键异构酶家族。它在乳腺癌、卵巢癌、前列腺癌和肝癌中表达，与肿瘤的分化程度、增殖速度和预后相关。研究显示，AGR3在乳腺癌患者中表达与更长的无进展生存期和更好的预后相关。其具体作用机制尚在研究中，但可能与蛋白质折叠和巯基-二硫化物交换反应有关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒可应用于科研领域中对AGR3蛋白表达量的精准检测，适用于肿瘤发生机制研究、疾病相关分子标志物筛选、药物干预效果评估等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AG-3 ELISA Kit; AG3 ELISA Kit; Agr3 ELISA Kit; AGR3_HUMAN ELISA Kit; Anterior gradient 3; xenopus; homolog of ELISA Kit; Anterior gradient homolog 3 ELISA Kit; Anterior gradient homolog 3 (Xenopus laevis) ELISA Kit; anterior gradient protein 3 ELISA Kit; Anterior gradient protein 3 homolog ELISA Kit; BCMP11 ELISA Kit; Breast cancer membrane protein 11 ELISA Kit; Gm888 ELISA Kit; hAG 3 ELISA Kit; hAG-3 ELISA Kit; HAG3 ELISA Kit; OTTHUMP00000158512 ELISA Kit; PDIA18 ELISA Kit; Protein disulfide isomerase family A member 18 ELISA Kit
缩写：AGR3
Uniprot No.：Q8TD06
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AGR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human AGR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7132.6622.6882.51552.3762.4312.4042.2312.51.9342.0011.9681.7951.251.5091.5821.5461.3730.6250.9741.0200.9970.8240.3120.5640.5590.5620.3890.1560.2960.2790.2880.11500.1780.1670.173 | ||||||
货期：3-5 working days
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