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产品描述：人前梯度蛋白2同系物(AGR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGR2含量。AGR2是重要靶点。它参与多种生物学过程，在肿瘤发生、发展、侵袭及转移等方面有作用。研究发现其表达异常与肿瘤恶性程度等相关，通过调节细胞增殖、凋亡等机制发挥影响，有望成为肿瘤诊断、治疗的关键靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础医学研究中AGR2蛋白表达水平的定量分析，例如肿瘤生物学研究、炎症相关疾病模型构建、细胞信号通路解析以及生物标志物筛选等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AG 2 ELISA Kit; AG 2 protein ELISA Kit; AG-2 ELISA Kit; AG2 ELISA Kit; AG2 protein ELISA Kit; AGR 2 ELISA Kit; AGR2 ELISA Kit; AGR2_HUMAN ELISA Kit; Anterior gradient 2 homolog (Xenopus laevis) ELISA Kit; anterior gradient 2 homolog ELISA Kit; Anterior gradient 2; protein disulphide isomerase family member ELISA Kit; Anterior gradient 2; Xenopus; homolog of ELISA Kit; Anterior gradient homolog 2 ELISA Kit; Anterior gradient protein 2 homolog ELISA Kit; Epididymis secretory protein Li 116 ELISA Kit; GOB 4 ELISA Kit; GOB4 ELISA Kit; HAG 2 ELISA Kit; hAG-2 ELISA Kit; hAG2 ELISA Kit; HAG2/R ELISA Kit; HEL S 116 ELISA Kit; HPC 8 ELISA Kit; hPC8 ELISA Kit; PDIA17 ELISA Kit; Protein disulfide isomerase family A member 17 ELISA Kit; secreted cement gland homolog ELISA Kit; Secreted cement gland protein XAG 2 homolog ELISA Kit; Secreted cement gland protein XAG-2 homolog ELISA Kit; Secreted cement gland protein XAG2 homolog ELISA Kit; XAG 2 ELISA Kit; XAG2 ELISA Kit
缩写：AGR2
Uniprot No.：O95994
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AGR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-110 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-90 | |||||
回收率：
| The recovery of human AGR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-100 | ||||
| EDTA plasma (n=4) | 87 | 81-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5012.4272.4642.29651.9432.0752.0091.8412.51.4271.4621.4451.2771.250.9010.9450.9230.7550.6250.6100.5840.5970.4290.3120.4110.4300.4210.2530.1560.2910.3040.2980.13000.1650.1710.168 | ||||||
货期：3-5 working days
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