首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人晚期糖基化终末产物(AGEs)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGEs含量。AGEs即晚期糖基化终末产物，在高糖环境等条件下大量生成并在体内蓄积。其背景与糖尿病、衰老等多种病理过程相关。研究机制上，AGEs可与受体相互作用，激活多种信号通路，引发氧化应激和炎症反应，导致组织损伤和功能障碍。试剂盒检测范围为0.78 μg/mL-50 μg/mL，本试剂盒基于特异性抗体对捕获和检测靶标，适用于科研场景中AGEs相关机制研究，例如糖尿病模型动物血清/组织样本分析、体外药物干预对AGEs生成抑制效果的评估，或阿尔茨海默病等神经退行性疾病中AGEs沉积的病理学关联探索。支持科研人员高效开展AGEs在代谢性疾病、慢性炎症或衰老机制中的基础研究与体外实验数据获取。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AGEs
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 μg/mL-50 μg/mL
灵敏度：0.195 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-109 | |||||
回收率：
| The recovery of human AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µg/mlOD1OD2AverageCorrected502.6982.6522.6752.534252.0762.0822.0791.93812.51.2041.2831.2441.1036.250.6390.6690.6540.5133.120.3880.3740.3810.2401.560.2610.2590.2600.1190.780.1870.1790.1830.04200.1450.1370.141 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human AGEs antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AGEs antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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