首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠不对称二甲基精氨酸(ADMA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADMA含量。ADMA（非对称二甲基精氨酸）是一种内源性一氧化氮合酶抑制剂，其水平升高与多种疾病如糖尿病视网膜病变和骨关节炎相关。研究显示，ADMA通过影响细胞外基质沉积、SOX9降解等途径，促进疾病发展。靶向DDAH1（ADMA水解酶）可能成为治疗相关疾病的新策略。试剂盒检测范围为1.5 ng/mL-300 ng/mL，本试剂盒适用于心血管疾病模型研究、代谢紊乱机制探索、肾脏功能评估等基础科研实验，也可用于药物干预或基因调控实验中ADMA水平变化的动态监测，为动物模型病理机制解析及治疗策略开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ADMA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.5 ng/mL-300 ng/mL
灵敏度：0.75 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 99-106 | |||||
回收率：
| The recovery of rat ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-104 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 3000.2010.2040.203 750.3170.3450.331 240.5230.5290.526 60.9520.9030.928 1.51.3811.3161.349 02.2902.2122.251 | ||||||
货期：3-5 working days
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