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产品描述：犬肾上腺髓质素(ADM)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的ADM含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验环境下探索ADM在犬类疾病模型中的调控机制、药物干预研究或基础生理功能解析，为动物医学及比较医学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADM ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
缩写：ADM
Uniprot No.：O77559
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of dog ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-104 | ||||
| EDTA plasma (n=4) | 97 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6752.6412.6582.5025002.1592.1442.1521.9962501.3121.3101.3111.1551250.7780.7880.7830.62762.50.3770.3600.3690.21331.250.2760.2640.2700.11415.60.1900.1950.1930.03700.1540.1580.156 | ||||||
货期：3-5 working days
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