首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肾上腺髓质素(ADM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADM含量。试剂盒检测范围为0.312 pg/mL-20 pg/mL，适用于基础科研中探索ADM在心血管疾病模型、代谢调控或炎症相关信号通路中的作用；为体外研究ADM在体液或组织中的表达分布、动态变化及其分子调控机制提供可靠工具，广泛应用于细胞实验、动物模型或疾病机制研究等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adm ELISA Kit; ADM precursor ELISA Kit; ADML_HUMAN ELISA Kit; Adrenomedullin ELISA Kit; AM ELISA Kit; PAMP ELISA Kit; Preproadrenomedullin ELISA Kit; Proadrenomedullin N-20 terminal peptide ELISA Kit; Proadrenomedullin N20 terminal peptide ELISA Kit; ProAM N terminal 20 peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; ProAM N20 ELISA Kit; ProAM-N20 ELISA Kit; ProAMN20 ELISA Kit
缩写：ADM
Uniprot No.：P35318
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 pg/mL-20 pg/mL
灵敏度：0.078 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 96 | ||||
| Range % | 90-104 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 86-98 | |||||
| 1:20 | Average % | 88 | ||||
| Range % | 80-95 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 88-100 | |||||
回收率：
| The recovery of human ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected202.3142.1312.2232.025101.9231.9021.9131.71551.4281.3811.4051.2072.51.0030.9920.9980.8001.250.7490.7310.7400.5420.6250.5280.5090.5190.3210.3120.3020.2940.2980.10000.1990.1970.198 | ||||||
货期：3-5 working days
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