Mouse adrenomedullin,ADM ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E10061m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠肾上腺髓质素(ADM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ADM含量。腺苷酸脱氨酶（ADM）是机体内重要的血管活性肽，具有舒张血管、降低血压、抑制血管平滑肌细胞增殖和迁移等作用。其研究机制主要聚焦于与受体结合后激活下游信号通路，调控细胞功能，在心血管疾病、肿瘤等多种疾病中展现潜在治疗价值。试剂盒检测范围为4.7 pg/mL-300 pg/mL，该产品适用于心血管疾病动物模型研究、高血压机制探索、肾脏生理功能研究及炎症相关信号通路分析等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Adm ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
缩写：
ADM
Uniprot No.：
P97297
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, cell culture supernates, tissue homogenates
检测范围：
4.7 pg/mL-300 pg/mL
灵敏度：
1.2 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-94
1:2	Average %	92
	Range %	88-96
1:4	Average %	101
	Range %	96-107
1:8	Average %	92
	Range %	87-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
300	2.534	2.452	2.493	2.352
150	2.234	2.176	2.205	2.064
75	1.804	1.875	1.840	1.699
37.5	1.276	1.239	1.258	1.117
18.75	0.745	0.724	0.735	0.594
9.4	0.419	0.433	0.426	0.285
4.7	0.250	0.239	0.245	0.104
0	0.137	0.144	0.141