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产品描述：牛脂联素(ADP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADIPOQ含量。ADIPOQ编码脂联素，是脂肪组织分泌的一种蛋白质。它具有抗炎、改善胰岛素敏感性等作用，与代谢综合征、心血管疾病等密切相关。研究机制主要围绕其信号通路展开，如激活AMPK等，以调节糖脂代谢，为相关疾病治疗带来新思路。试剂盒检测范围为3.125 μg/mL-50 μg/mL，适用于科研领域中对动物代谢调控机制、营养干预效果或疾病模型的评估，为肥胖相关研究、代谢性疾病动物模型构建等提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADIPOQ ELISA kit; ACRP30 ELISA kit; APM1Adiponectin ELISA kit; 30 kDa adipocyte complement-related protein ELISA kit; Adipocyte complement-related 30 kDa protein ELISA kit; ACRP30 ELISA kit; Adipocyte ELISA kit; C1q and collagen domain-containing protein ELISA kit; Adipose most abundant gene transcript 1 protein ELISA kit; apM-1 ELISA kit
缩写：ADIPOQ
Uniprot No.：Q3Y5Z3
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：3.125 μg/mL-50 μg/mL
灵敏度：3.125 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-91 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average500.2120.2070.210250.3450.3320.33912.50.5890.6070.5986.251.0771.1061.0923.1252.2142.1232.169 | ||||||
货期：3-5 working days
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