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产品描述：仓鼠脂联素，含C1Q和胶原域(ADIPOQ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADIPOQ含量。ADIPOQ是脂联素基因。脂联素由脂肪组织分泌，具有抗糖尿病、抗炎、抗动脉粥样硬化等作用。研究机制上，它可与多种受体结合，激活下游信号通路，调节能量代谢、改善胰岛素敏感性，在代谢性疾病等研究中受关注。试剂盒检测范围为0.469 ng/mL-30 ng/mL，该产品适用于科研实验中评估仓鼠模型在代谢疾病干预、脂肪组织功能研究、运动生理学或药物开发过程中ADIPOQ表达水平的变化，为探索脂联素在能量代谢调控机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ADIPOQ
Uniprot No.：C9DFG2
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, tissue homogenates
检测范围：0.469 ng/mL-30 ng/mL
灵敏度：0.117 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 85 | ||||
| Range % | 82-90 | |||||
| 1:2000 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8000 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-92 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.7032.7682.7362.595152.2442.2012.2232.0827.51.6881.6981.6931.5523.751.0731.0541.0640.9231.8750.6110.6350.6230.4820.9380.3210.3420.3320.1910.4690.2150.2300.2230.08200.1400.1420.141 | ||||||
货期：3-5 working days
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