首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人乙醇脱氢酶(ADH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的ADH1A含量。ADH1A是一个重要靶点。它在乙醇代谢等生理过程中发挥关键作用。研究机制方面，聚焦于其基因表达调控及蛋白功能。通过探究它与疾病的关联，如酒精性肝病等，为开发针对性治疗药物和干预策略提供理论依据和研究方向。试剂盒检测范围为0.312 mU/mL-20 mU/mL，适用于酒精代谢机制研究、肝损伤模型评估、药物或化合物对ADH活性调控作用分析等科研场景，特别针对体外培养细胞或动物实验样本中的ADH活性检测需求开发，为代谢组学、毒理学及肝脏病理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADH alpha subunit ELISA Kit; ADH ELISA Kit; ADH1 ELISA Kit; ADH1A ELISA Kit; ADH1A_HUMAN ELISA Kit; Alcohol dehydrogenase 1 (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; Alcohol dehydrogenase 1A (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1A ELISA Kit; Alcohol dehydrogenase subunit alpha ELISA Kit; Aldehyde reductase ELISA Kit
缩写：ADH1A
Uniprot No.：P07327
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.312 mU/mL-20 mU/mL
灵敏度：0.078 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 83 | ||||
| Range % | 80-87 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 83-89 | |||||
回收率：
| The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 99-105 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected202.2942.1562.2252.107101.7781.6981.7381.62051.2531.2401.2471.1292.50.7970.7750.7860.6681.250.4850.4680.4770.3590.6250.3620.3750.3690.2510.3120.2560.2620.2590.14100.1190.1170.118 | ||||||
货期：3-5 working days
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