首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠乙醇脱氢酶(ADH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADH含量。ADH即抗利尿激素，它主要由下丘脑视上核和室旁核的神经内分泌细胞合成。其作用机制主要是增加肾小管对水的重吸收，以维持机体水平衡。在临床上，ADH水平异常会引发如尿崩症等疾病，相关研究有助于深入了解水代谢调节机制与疾病治疗。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究乙醇代谢调控机制、评估肝损伤模型中ADH表达水平动态变化，或探究药物干预对酶活性影响的实验场景；为研究乙醇代谢通路、肝脏功能评估及毒性物质作用机制提供高效的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adh1 ELISA Kit; Adh-1 ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; EC 1.1.1.1 ELISA Kit; Alcohol dehydrogenase A subunit ELISA Kit
缩写：ADH
Uniprot No.：P06757
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 96-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-99 | ||||
| EDTA plasma (n=4) | 88 | 80-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3122.4562.3842.237101.5861.7121.6491.50251.0091.0541.0320.8852.50.6410.6320.6370.4901.250.4120.4350.4240.2770.6250.2870.2740.2810.1340.3120.2110.2180.2150.06800.1460.1470.147 | ||||||
货期：3-5 working days
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