首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脂肪分化相关蛋白(ADRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的ADFP含量。ADFP 即脂肪分化相关蛋白，是一种与脂质代谢密切相关的蛋白质。它在多种细胞中表达，参与脂质小滴的形成、维持和代谢调节。研究发现其异常表达与肥胖、脂肪肝等代谢性疾病的发生发展相关，目前正深入探究其在疾病发生机制中的具体作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于脂代谢相关疾病机制研究、脂肪细胞分化模型评估、药物干预效果分析等科研领域，为探索肥胖、非酒精性脂肪肝等代谢性疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADFP ELISA Kit; Adipophilin ELISA Kit; Adipose differentiation related protein ELISA Kit; Adipose differentiation-related protein ELISA Kit; ADRP ELISA Kit; MGC10598 ELISA Kit; Perilipin-2 ELISA Kit; PLIN2 ELISA Kit; PLIN2_HUMAN ELISA Kit
缩写：ADFP
Uniprot No.：Q99541
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-110 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human ADRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.6942.7522.7232.602 1002.0822.0162.0491.928 501.4181.4041.4111.290 250.8910.8390.8650.744 12.50.5400.5240.5320.411 6.250.3500.3590.3550.234 3.120.2760.2690.2730.152 00.1240.1170.121 | ||||||
货期：3-5 working days
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