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产品描述：小鼠解整合素金属蛋白酶17(ADAM17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAM17含量。ADAM17 是重要的靶点。它是一种膜整合糖蛋白，具有金属蛋白酶活性。在生理上参与调节细胞信号传导、细胞黏附等。研究发现其异常活化与多种疾病如炎症、肿瘤等密切相关，通过对其作用机制的研究，有望开发针对性治疗药物。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验中对小鼠模型血清、血浆样本的ADAM17含量分析，或组织样本（如肿瘤、炎症部位）中蛋白酶活性相关的分子机制研究，为探索ADAM17在免疫调控、肿瘤微环境及药物干预效果评估等领域的科研应用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adam17 ELISA kit; TaceDisintegrin and metalloproteinase domain-containing protein 17 ELISA kit; ADAM 17 ELISA kit; EC 3.4.24.86 ELISA kit; TNF-alpha convertase ELISA kit; TNF-alpha-converting enzyme ELISA kit; CD antigen CD156b ELISA kit
缩写：ADAM17
Uniprot No.：Q9Z0F8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-109 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of mouse ADAM17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 89 | 83-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4042.6012.5032.3355001.6321.5891.6111.4432500.9770.9890.9830.8151250.6060.5880.5970.42962.50.3560.3420.3490.18131.250.2580.2780.2680.10015.60.2020.2150.2090.04100.1610.1740.168 | ||||||
货期：3-5 working days
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