首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠活化素B(ACV-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACV-B含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于实验室研究，可广泛用于评估动物模型中ACV-B在卵巢功能、胚胎形成、代谢综合征（如多囊卵巢或肥胖模型）中的表达变化，亦可用于探索其在组织再生或炎症反应中的调控机制。实验兼容多种生物样本类型，为体外研究提供灵活选择本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACV-B
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-104 | |||||
回收率：
| The recovery of mouse ACV-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 90-95 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.2782.2522.2652.1792002.1322.0152.0741.9881001.6121.6411.6271.541500.9870.9330.9600.874250.5410.5220.5320.44612.50.2780.2850.2820.1966.250.1840.1940.1890.10300.0850.0870.086 | ||||||
货期：3-5 working days
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