首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠促肾上腺皮质激素(ACTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTH含量。促肾上腺皮质激素（ACTH）是腺垂体分泌的调节肾上腺皮质活动的重要激素。其分泌受下丘脑促肾上腺皮质激素释放激素调控。研究机制方面，它作用于肾上腺皮质，刺激皮质醇等糖皮质激素合成与分泌，在应激反应、代谢调节等生理过程中发挥关键作用。试剂盒检测范围为1.25 pg/mL-50 pg/mL，适用于评估实验大鼠应激状态下的激素水平变化、肾上腺皮质功能研究及药物干预对神经内分泌系统的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ACTH
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 pg/mL-50 pg/mL
灵敏度：1.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of rat ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-96 | ||||
| EDTA plasma (n=4) | 101 | 97-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 500.3410.3400.341 250.4760.4740.475 11.50.7000.7130.707 3.751.0561.0671.062 1.251.4681.3871.428 01.9451.8641.905 | ||||||
货期：3-5 working days
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