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产品描述：人α-辅肌动蛋白4(ACTN-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACTN4含量。ACTN4是一个重要靶点。它在细胞的多种生理活动中发挥关键作用，与细胞骨架组装、细胞运动等密切相关。在研究机制上，主要聚焦于其在疾病发生发展中的功能影响，如在肿瘤等疾病里的作用机制探索，以寻找潜在治疗策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探索ACTN - 4在疾病发生发展中的作用机制、评估实验模型中蛋白表达水平变化，或作为生物标志物筛选的工具，为细胞生物学、病理学及药物研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：actinin 4 ELISA Kit; Actinin alpha 4 ELISA Kit; actinin4 ELISA Kit; ACTN 4 ELISA Kit; ACTN4 ELISA Kit; ACTN4_HUMAN ELISA Kit; Alpha-actinin-4 ELISA Kit; DKFZp686K23158 ELISA Kit; F actin cross linking protein ELISA Kit; F-actin cross-linking protein ELISA Kit; Focal segmental glomerulosclerosis 1 ELISA Kit; FSGS 1 ELISA Kit; FSGS ELISA Kit; FSGS1 ELISA Kit; Non muscle alpha actinin 4 ELISA Kit; Non-muscle alpha-actinin 4 ELISA Kit
缩写：ACTN4
Uniprot No.：O43707
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ACTN-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-105 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human ACTN-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 99 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.2562.2982.2772.130 10001.9641.9041.9341.787 5001.4331.4671.4501.303 2501.0211.0611.0410.894 1250.6980.6550.6770.530 62.50.4780.4910.4850.338 31.250.2670.2830.2750.128 00.1460.1480.147 | ||||||
货期：3-5 working days
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